LOCUS       40924_7711       10564 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pB[Lchsp24-pdgf-b], complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10564)
  AUTHORS   Linger RJ, Belikoff EJ, Yan Y, Li F, Wantuch HA, Fitzsimons HL, 
            Scott MJ.
  TITLE     Towards next generation maggot debridement therapy: transgenic 
            Lucilia sericata larvae that produce and secrete a human growth 
            factor
  JOURNAL   BMC Biotechnol. 16 (1), 30 (2016)
  PUBMED    27006073
REFERENCE   2  (bases 1 to 10564)
  AUTHORS   Linger RJ, Belikoff EJ, Yan Y, Li F, Wantuch HA, Fitzsimons HL, 
            Scott MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2015) Entomology, North Carolina State University,
            112 Derieux Place, Raleigh, NC 27695-7614, USA
REFERENCE   3  (bases 1 to 10564)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10564)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016"; volume: "16"; issue: "1"; pages: "30"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-OCT-2015) Entomology, North Carolina State University, 112 
            Derieux Place, Raleigh, NC 27695-7614, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     mobile_element  1084..1858
                     /mobile_element_type="transposon:pBAC"
                     /label=BAC
     regulatory      1878..3036
                     /label=Lchsp24
                     /note="Lchsp24"
                     /regulatory_class="promoter"
     5'UTR           2895..3074
     CDS             3075..3464
                     /codon_start=1
                     /product="prePDGF-B"
                     /label=prePDGF-B
                     /protein_id="AMQ78449.1"
                     /translation="MKSFLLVLFAFLAVFAFVQASLGSLTIAEPAMIAECKTRTEVFEI
                     SRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIF
                     KKATVTLEDHLACKCETVAAARPVT"
     regulatory      3513..4048
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    3513..4048
                     /label=Lchsp70
                     /note="Lchsp70"
     regulatory      4055..6263
                     /label=Lchsp83
                     /note="Lchsp83"
                     /regulatory_class="promoter"
     CDS             6264..6956
                     /label=ZsGreen
                     /note="Zoanthus green fluorescent protein (Matz et al.,
                     1999)"
     regulatory      6966..7432
                     /label=Lctub pA
                     /note="Lctub pA"
                     /regulatory_class="polyA_signal_sequence"
     mobile_element  7483..8160
                     /mobile_element_type="transposon:pBAC"
                     /label=BAC
     primer_bind     complement(8343..8359)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8367..8383
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8391..8421)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8436..8457)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8745..9333)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9507..10364)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10365..10469)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctttgttta aaatataaca
      421 aaattgtgat cccacaaaat gaagtggggc aaaatcaaat aattaactag tgtccgtaaa
      481 cttgttggtc ttcaactttt tgaggaacac gttggacggc aaatcgtgac tataacacaa
      541 gttgatttaa taattttagc caacacgtcg ggctgcgtgt tttttgcgct ctgtgtacac
      601 gttgattaac tggtcgatta aataatttaa tttttggttc ttctttaaat ctgtgatgaa
      661 attttttaaa ataactttaa attcttcatt ggtaaaaaat gccacgtttt gcaacttgtg
      721 agggtctaat atgaggtcaa actcagtagg agttttatcc aaaaaagaaa acatgattac
      781 gtctgtacac gaacgcgtat taacgcagag tgcaaagtat aagagggtta aaaaatatat
      841 tttacgcacc atatacgcat cgggttgata tcgttaatat ggatcaattt gaacagttga
      901 ttaacgtgtc tctgctcaag tctttgatca aaacgcaaat cgacgaaaat gtgtcggaca
      961 atatcaagtc gatgagcgaa aaactaaaaa ggctagaata cgacaatctc acagacagcg
     1021 ttgagatata cggtattcac gacagcaggc tgaataataa aaaaattaga aactattatt
     1081 taaccctaga aagataatca tattgtgacg tacgttaaag ataatcatgc gtaaaattga
     1141 cgcatgtgtt ttatcggtct gtatatcgag gtttatttat taatttgaat agatattaag
     1201 ttttattata tttacactta catactaata ataaattcaa caaacaattt atttatgttt
     1261 atttatttat taaaaaaaaa caaaaactca aaatttcttc tataaagtaa caaaactttt
     1321 aaacattctc tcttttacaa aaataaactt attttgtact ttaaaaacag tcatgttgta
     1381 ttataaaata agtaattagc ttaacttata cataatagaa acaaattata cttattagtc
     1441 agtcagaaac aactttggca catatcaata ttatgctctc gacaaataac ttttttgcat
     1501 tttttgcacg atgcatttgc ctttcgcctt attttagagg ggcagtaagt acagtaagta
     1561 cgttttttca ttactggctc ttcagtactg tcatctgatg taccaggcac ttcatttggc
     1621 aaaatattag agatattatc gcgcaaatat ctcttcaaag taggagcttc taaacgctta
     1681 cgcataaacg atgacgtcag gctcatgtaa aggtttctca taaatttttt gcgactttga
     1741 accttttctc ccttgctact gacattatgg ctgtatataa taaaagaatt tatgcaggca
     1801 atgtttatca ttccgtacaa taatgccata ggccacctat tcgtcttcct actgcagggt
     1861 aaccattcta gactcgagtc attttttgtt atttgttatt agggtgggca attttttcta
     1921 atgcccatta cccttagaat taagatgata tacaaaaaag ttctttttgc tttacgattt
     1981 cgaatataaa tctactgtta atttaagttc tagcccaatt cttattcagt tgtagttttg
     2041 ttctagttca gttctagttc agttctagtt ccgttctagt tcagttctag ttcagttcta
     2101 gttcagttct agttcagttc tagttcagtt ctagttcagt tctagttcag ttctagttca
     2161 gttctagttc agttctagtt cagttctagt tcagttctag ttcagttcta gttcagttct
     2221 agttcagttc tagttcagtt ctagttcagt tctagttcag ttctagttca gttctagttc
     2281 agttctagtt cagttctagt tcagttctag ttcagttcta gttcagttct agttcagttc
     2341 tagttcagtt ctagttcagt tctagttcag ctctagttca gctctagttc agttctagtt
     2401 cagttctagt tcagttgtag ttcagttcta gtacagttct agttcagttc tagtttagtt
     2461 ctagttcagt tcatgttcag ttctcgttca gtactagttc agttctagtt caattcagtt
     2521 actgtttagt tctagttcag ttctagttca gttctagttt agttctagtt cagttctatt
     2581 ttaatataaa tcatataata atattattaa attttaatta aaaaatttta ataattaaaa
     2641 ataaaaattt acgagaaaaa tataaaattt gcaaaaactc cttaattgcg cagatttact
     2701 ctcttaattt ttgagaagcc aataaaaatt ttgagaaaaa taagttttga aaaacacaca
     2761 ttaaccactc tctttttaag agagtacttc acaatcaata tttaagagaa acaaattaaa
     2821 agttttcgag aatttactcg aaaaaatctt aacaagtttt ttgttataaa agccggcaaa
     2881 acacagaagg cgtcatcagt tgaacaaata attacagaaa gtgcggataa agcgtgaaaa
     2941 tattgtaaaa actttcaaaa gttaaaaaaa ctaagcaaac agaaataaaa tcttttttaa
     3001 ttaaataaaa ctaaacgttt taagaaaacc aaagagggat cccggtcgat cggctaggta
     3061 ccggatccgc caccatgaag tcgttcttgt tggtgttgtt cgccttcttg gccgtttttg
     3121 ccttcgttca agcctcattg ggctcattga ccattgctga acccgccatg attgccgaat
     3181 gtaaaacccg taccgaagtg ttcgaaattt cccgtcgttt gattgatcgt accaatgcca
     3241 atttcttggt ttggcccccc tgtgttgaag tgcaacgttg tagtggttgt tgtaataatc
     3301 gtaatgtgca atgtcgtccc acccaagttc aattgcgtcc cgtgcaagtt cgtaagattg
     3361 aaattgtgcg taaaaagccc attttcaaga aggccaccgt taccttggaa gatcatttgg
     3421 cctgtaaatg tgaaactgtt gctgccgccc gtcccgtgac ctaatgaaag cttgagctca
     3481 tggcgcgcct agggtttaaa ctccggaaag cttagtcaat ctcaatttca ttcctagaaa
     3541 caattcatca ttaaaactca tgaatattgt tcatttagaa taagaaataa tttattttta
     3601 gccaataaga tctcaaataa agagaaatga ataaattgtt ttatgttaaa acaaatatgt
     3661 aaattataac aaaatgtgtt ttattaaccc acacaaaaac aaagatgaat tgaatttgta
     3721 tatatctaaa atttaagagt gtgataaata tttttggggg tccttaagtc tgtttctaac
     3781 aaaaattctt ccaaaacatt cattggttac aagaattttt tggtactcat gatcacagtt
     3841 tgcaaaaaat ctttttttaa gaatatttgt atatttatgg actgattttt ctaattttaa
     3901 tagcgttctt tctgaacatt agaactattc ggattttgca gatatcaggg gttctgtgaa
     3961 aacagattta aaaaaagtga gtgtgaaaat agtattatag ggaattacaa acttatatgt
     4021 acatatcctt gtatatatca ttctcgaggc ggcccacgtg ttgaaaccat tatcagaaaa
     4081 taattcactt aaacattata atttgataaa aagaacacta aaacaaaata ttataattat
     4141 accgccaaaa attcaacaaa ctttaagaaa tttcgagtaa attgcaccgc aaactaaaaa
     4201 accgacatga taaacaaaaa aaatacataa aagagcgcaa acagctgtca tttacagggt
     4261 tgtagatatt tgaaattttt acctaaaaag gggaaaataa aaggccacac ttttggaaat
     4321 tacaaatttg tgagagaatt tttgatttca tttattaaat ttatataaaa acaaaagagt
     4381 tgttataata tgtatgagat aatgtttgca aattttaatt tttaaatttt tacagtcaaa
     4441 ttttataaaa tatgttaaaa gtatatgatt gtcacatttt taaattattc cacttttcat
     4501 tgtttttatt caagaccggc aactctgatg ttttgctgca catcagctgc actggcgaaa
     4561 aaattattaa tgcccccatg atgagagaaa atcaatattt tttcatttta tgcattttta
     4621 taaattttgt tagcaatatt attgttttaa taatgttatt attttcttta ataaaacaat
     4681 taaattactt tcatttataa tttatgatga ctaatatgta aatcgaatgt gattaaatca
     4741 tacatactat ttccatataa agcacaaagt ttaatatata attaatatta aatttattta
     4801 atttaaaaat taatagttaa tacatatttc aattaatcaa atacatatta agcttgtaac
     4861 ttagttcaaa aaacacttaa ttatacatag atttgtcaat taataagact ttttaactat
     4921 aatttaaaga ataaaacgtt tttgaaataa ataagttcat tgtttgacta gaaaaatatt
     4981 tagtttatgt catgatatta ctcagcaatg cctaagatta attccttttt catttaatta
     5041 ataactttta tatatttttg cataaataaa taaagtaaat tatagttgat tcattccgca
     5101 tgttattaaa aacagagaga acattgtaga aattttgaca ttgatgcaat tttacagttt
     5161 aaaagccact gcttctacat atatacaacg ctgcgtgggc attaatcatg ttgaatgttt
     5221 tgcacatttt tttgataatt tgcaactaat aaattaaaat aacaataaaa acctataatt
     5281 aatattggtt ttctacaatt ttaataaatt aattttttta tatatctaaa atgtaaccaa
     5341 tttcttttgt tatcaccaaa taaacaacca catgatgctg tttagagcct acggaagtac
     5401 gggtagtttg ttccagaaca ttctagtatt ttcgtttatt tttgttttac ctataaattc
     5461 agtgcatggc tgctaaccgg catcagtctt gaaagaaaaa ttgtgcaaaa aacaccaaga
     5521 attccaaacg ggagaaagtt aaaagtgttt taattttctt aataaagaaa agtctgaaaa
     5581 aagaacatta aaaaagtgtg tagttgttta atttcaattg caaaaagaaa aataatttaa
     5641 aaagtattta tttttgtgaa attaatataa aacaaacaca tcaaggcaag tttttattaa
     5701 aaaaaataaa atgtagtaac aaaacgtttt tgttcttttg aaaaaagtgt gatgtaaaaa
     5761 ctcaggtaat ttgccagaaa gaaaaacctt caatatggtt taagaataac gttactaagt
     5821 aattgtaaat tgttctagat taaattttct ttggccatga catttttaga aaataaatct
     5881 agtatataaa gaaaatttca ccatgctaat aaaaagaaat ttctagcatg cgatttttta
     5941 aagaaaattt ttcaaaattt tttcgaaatt ataatcgata tttcatgttt tcattcgtta
     6001 aaaagcatac ccaatccacc cctaaaaaac tagtaattct taacttaaag agatttctag
     6061 atttttatta aaaattttca gtaccaatcc cactccctac acttaacgaa aaacgtgcgt
     6121 cattaaaatt atttatactt catattttga ttgattattt tactaataat ttgtattttt
     6181 ctattttttt tttattaaac acagatgcct gaagaagttg aaacttttgc ttccaagctg
     6241 aaattgctca gcttaaagga tccatggctc agtcaaagca cggtctaaca aaagaaatga
     6301 caatgaaata ccgtatggaa gggtgcgtcg atggacataa atttgtgatc acgggagagg
     6361 gcattggata tccgttcaaa gggaaacagg ctattaatct gtgtgtggtc gaaggtggac
     6421 cattgccatt tgccgaagac atattgtcag ctgcctttat gtacggaaac agggttttca
     6481 ctgaatatcc tcaagacata gctgactatt tcaagaactc gtgtcctgct ggttatacat
     6541 gggacaggtc ttttctcttt gaggatggag cagtttgcat atgtaatgca gatataacag
     6601 tgagtgttga agaaaactgc atgtatcatg agtccaaatt ttatggagtg aattttcctg
     6661 ctgatggacc tgtgatgaaa aagatgacag ataactggga gccatcctgc gagaagatca
     6721 taccagtacc taagcagggg atattgaaag gggatgtctc catgtacctc cttctgaagg
     6781 atggtgggcg tttacggtgc caattcgaca cagtttacaa agcaaagtct gtgccaagaa
     6841 agatgccgga ctggcacttc atccagcata agctcacccg tgaagaccgc agcgatgcta
     6901 agaatcagaa atggcatctg acagaacatg ctattgcatc cggatctgca ttgccctgaa
     6961 agcttgcagc caaacccatc atcgacaacc atcaaaacca aaaaaaaaag acagaagaag
     7021 tgtgtattga aaaaattctc gacctattag ctgaaactac tcaactaaac attcaatata
     7081 ctttttttta aacaaactgt tattttaaaa atgataacga atccaaaaca aaacacttgt
     7141 aaattttttt tattttcaaa tttgaaaaaa acatgaatga aaagaacaaa ttttaaagaa
     7201 atgcacttta aaaacacgaa aaaaaaacaa gaacctaata aaatgaaagg cttaagctgc
     7261 attgaatcat aatattccaa aagaaaattt tttaatgttt tactacttaa tggggggaaa
     7321 aagagggaaa acaactaaaa atagtaattg ttaaaatgct tggcaaaagt ttaatgaact
     7381 tgccgaggtg tagaggaaac tataaaaatg catagtgagt gcattaaaaa cacactagtt
     7441 ctagagcggc cgctcgaccc gcggccatgg aggcctggta ccagatctga caatgttcag
     7501 tgcagagact cggctacgcc tcgtggactt tgaagttgac caacaatgtt tattcttacc
     7561 tctaatagtc ctctgtggca aggtcaagat tctgttagaa gccaatgaag aacctggttg
     7621 ttcaataaca ttttgttcgt ctaatatttc actaccgctt gacgttggct gcacttcatg
     7681 tacctcatct ataaacgctt cttctgtatc gctctggacg tcatcttcac ttacgtgatc
     7741 tgatatttca ctgtcagaat cctcaccaac aagctcgtca tcgctttgca gaagagcaga
     7801 gaggatatgc tcatcgtcta aagaactacc cattttatta tatattagtc acgatatcta
     7861 taacaagaaa atatatatat aataagttat cacgtaagta gaacatgaaa taacaatata
     7921 attatcgtat gagttaaatc ttaaaagtca cgtaaaagat aatcatgcgt cattttgact
     7981 cacgcggtcg ttatagttca aaatcagtga cacttaccgc attgacaagc acgcctcacg
     8041 ggagctccaa gcggcgactg agatgtccta aatgcacagc gacggattcg cgctatttag
     8101 aaagagagag caatatttca agaatgcatg cgtcaatttt acgcagacta tctttctagg
     8161 gttaaaaaag atttgcgctt tactcgacct aaactttaaa cacgtcatag aatcttcgtt
     8221 tgacaaaaac cacattgtgg ccaagctgtg tgacgcgacg cgcgctaaag aatggcaaac
     8281 caagtcgcgc gagcgtcgac tctagaggat ccccgggtac cgagctcgaa ttcgtaatca
     8341 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8401 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8461 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8521 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8581 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8641 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8701 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8761 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8821 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8881 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     8941 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9001 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9061 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9121 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9181 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9241 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9301 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9361 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9421 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9481 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9541 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9601 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9661 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9721 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9781 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9841 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9901 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9961 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10021 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10081 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10141 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10201 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10261 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10321 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10381 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10441 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    10501 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    10561 cgtc
//
